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When high-speed train passes through the tunnel or meets each other, the air pressure fluctuation will be generated on the train surface. This fluctuation passes through the body gap and ventilation fan, air duct into the train, which will impact the air fluctuation inside of the train, and symptom of tinnitus or earache may appear, which has an impact on the travelling comfort. In order to restrain the fluctuation of pressure in the high-speed train, according to the characteristic curve of ventilator fan and equivalent leak relations of car body, the transfer relationship between inside and outside is built. The interior pressure and the interior pressure change rate are taken as the control input. The fuzzy algorithm is used to adjust the frequency of the fresh air fan and exhaust fan. The simulation results show that:this control method can improve the inhibition ability of the existing ventilation system and the travelling comfort.