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The stability test system of a certain type of aiming line is a special equipment used to test the independent performance of the photoelectric tracking subsystem of the team, such as the group weapon system, the lightlow altitude anti missile gun and so on. Introduces the realization of communication between PC and single axis turntable by using LabWindows/CVI, designs a line of sight stabilization test system, analysises the single axis turntable to physical quantity information of real-time detection.In order to improve the visibility and to achieve the control of the single axis turntable, data transmission through the RS232 serial communication technology. PC use LabWindows/CVI to control the widget to achieve data reception, data displaying and analysing. The experimental results show that the data acquisition and display system of RS232 serial communication based on LabWindows/CVI has the characteristics of strong visibility, simple operation and so on.