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With the large-scale formation and rapid development of Internet of things, the development of embedded system based on Ethernet communication has become a practical subject of application of Internet of things. This paper designed an Ethernet communication module with STM32F407 as the main processor. It used 32 bit flash microcontroller STM32F407 and Ethernet module CH395 to achieve serial communication. It was established data communication by using TCP/IP protocol stack built-in CH395 control chip and LabVIEW client. Experimental studies were obtained by sending test data in real time. The experimental results show that Ethernet communication embedded system based on STM32F407 was stable. Data transmission is reliable and it can meet system design requirements.