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With the development of science and technology, the semiconductor refrigeration technology has been widely used in military, medical, daily life and so on. However,in the existing refrigeration technology of the semiconductor refrigerator, since the hot end and the cold end cannot be separated like metal, there are problems like that the constant temperature effect is poor and the cooling efficiency is low and so on.To solve the above problems, according to the Peltier effect, a semiconductor refrigerator was designed using a push-pull power supply method and PID temperature control method,and three kinds of refrigeration strengths were designed: high grade, middle grade, and low grade.The final experimental results showed that, given the set temperature and refrigeration strength, the refrigerator can stabilize the temperature at the temperature set value after working for a period of time, and the fluctuation does not exceed 0.5℃ and the time used is in line with the expectation.