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In order to achieve the purpose of high-precision temperature field measurement, it is necessary to consider the comprehensive error caused by temperature sensor, connecting cable, conditioning amplifier, data acquisition system and environment, and design a set of whole loop in-situ calibration device for the temperature measurement system in the low temperature range. In this paper, the thermodynamic simulation calculation of the thermal insulation calibration chamber, which is the core part of the whole calibration device, is carried out. The three-layer structure of the main body, insulation technology, lead sealing mode, temperature field uniformity assurance measures are simulated and calculated. The test platform is established to verify the simulation results. The simulation and experimental results show that the design scheme of the whole calibration device of the cryogenic temperature measurement system can meet the requirements of 11 channels in one calibration, temperature accuracy of 50mk and effective temperature field uniformity of 50mk in the temperature range of 77K to 323k.