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To overcome the problem of low tracking and positioning accuracy caused by constantly changing moving targets, a high-precision positioning system for moving target tracking based on ultra wideband technology is designed. The image acquisition module consists of an FPGA unit, a VGA display unit, a frame buffer unit, and an image acquisition unit to achieve image acquisition in moving object tracking and positioning. In the ultra wideband technology module, the antenna, positioning base station, and mobile node required for ultra wideband moving object positioning are designed to complete ultra wideband dynamic networking and achieve hardware system design. Based on the images collected by the hardware system, implement image graying processing and morphological filtering processing to enhance the useful information in the image, design the TLD moving target tracking algorithm. As the moving target starts moving, the TLD model will continue to learn the tracking moving target, obtain the target changes in the distance, depth of field, angle and other levels, and continue to learn and identify to achieve good tracking results, design a dynamic positioning algorithm for moving targets based on ultra wideband technology, achieve high-precision positioning of moving targets based on tracking results, and complete the design of the software system. The experimental test results show that the tracking error rates of the system in medium and long distance target tracking and positioning experiments are less than 0.60% and 2.4%, and the positioning error at the bend of the route is relatively low when moving along the S-shape, which is in line with the flight path of the experimental moving target and has good positioning ability.